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- Title
COMPARISON OF POLYSACCHARIDES ISOLATED FROM THE MYCELIA OF A CULTIVATED STRAIN OF PORIA COCOS GROWN IN DIFFERENT LIQUID CULTURE MEDIA.
- Authors
Li-na Zhang; Li Chen; Yong Jin; Yu-lu Lin; Peter Chi Keung Cheung; Li-guo Chen
- Abstract
Mycelium of a cultivated strain of Poria cocos was grown by submerged fermentation in a liquid medium containing corn steep liquor with orbital shaking. Six polysaccharides coded as ac-PCMl, ac-PCM2, ac-PCM3- and II, acPCM4-I and II were isolated from the mycelium by extracting with 0.9% NaCl aqueous solution, hot water, 0.5 mol/L NaOH aqueous solution and 88% formic acid. Exo-polysaccharide was obtained from the culture medium and coded as ac-PCM0. The monosaccharide composition and molecular weights of these polysaccharides were characterized by using infrared spectroscopy, gas chromatography, elemental analysis, [SUP13]C-NMR, viscometry and light scattering. The results indicated that ac-PCM0, ac-PCM1 and ac-PCM2 are heteropolysaccharides containing glucose, galactose, mannose and fucose, and acPCM3-1 and ac-PCM3-II mainly consist of D-glucose. The content of the glucose in the polysaccharides increased with the isolation progress. Remarkably, α-glucan and β-glucan coexisted in the extract by NaOH aqueous solution (ac-PCM3), and could be separated by chemical methods. The protein in the ac-PCM polysaccharides cultured from the medium containing corn steep liquor was higher than in the ab-PCM from the medium with bran extract. Therefore, the polysaccharides from Poria cocos mycelia cultured in different media have different chemical composition, molecular weights and conformations.
- Subjects
MYCELIUM; PORIA cocos; FERMENTATION; POLYSACCHARIDES
- Publication
Chinese Journal of Polymer Science (World Scientific Publishing Company), 2003, Vol 21, Issue 4, p465
- ISSN
0256-7679
- Publication type
Article