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- Title
Saturating density of STSs (1/6 kb) in a 1.1 Mb region on 3q28-q29: a valuable resource for cloning of disease genes.
- Authors
Alexander, Christiane; Bernstein, Steven L; Rocchi, Mariano; Auburger, Georg
- Abstract
We have fine mapped 29 ESTs of Genemap'99 to YACs and radiation hybrids covering 8 cM of the chromosomal region of 3q28-q29. Focusing on the genetic interval of ∼1 Mb between markers D3S3669 and D3S3562 we established a sequence-ready PAC contig which covers the OPA1 locus containing the gene causing autosomal dominant optic atrophy (ADOA; OMIM[sup *]165500). The fidelity of the contig was increased by the generation of 181 PAC end sequences, 84 of which resulted in PCR-able STSs. Sequence content evaluation of the PAC ends by BLAST analysis identified two novel ESTs Iocalising to the OPA1 crucial interval.
- Subjects
MOLECULAR cloning; GENETIC markers
- Publication
European Journal of Human Genetics, 2001, Vol 9, Issue 4, p307
- ISSN
1018-4813
- Publication type
Article
- DOI
10.1038/sj.ejhg.5200630