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- Title
Antiviral activity of dermaseptin 01 against Dengue virus type 2, Herpes simplex virus type 1 and Vaccinia virus.
- Authors
De Souza Cardoso, João Luiz Macedo; Santos Soares, Maria José dos; Almeida Leite, José Roberto Souza; Cotta Malaquias, Luiz Cosme; Leomil Coelho, Luiz Felipe
- Abstract
Aims: To determine the in vitro antiviral activity of dermaseptin 01, an antimicrobial peptide discovered in the skin secretion of Phyllomedusa hypochondrialis frogs, against Herpes simplex virus type 1, Vaccinia virus and Dengue virus type 2. Methods: The peptide dermaseptin 01 was used for the cytotoxic assays using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay (MTT) in VERO, LLCMK2 and C6/36 cells. The antiviral activity was also evaluated using MTT for Herpes simplex virus type 1 and Vaccinia virus in VERO cells and for Dengue virus type 2 in LLCMK2 cells. The antiviral activity of dermaseptin 01was also evaluated in C6/36 cells infected with Dengue virus type 2 by cytopathic effect reduction. Results: The 50% cytotoxicity concentration of dermaseptin 01 was 105 μg/mL in insect cells (C6/36) and >1000 μg/mL in mammalian cells (VERO and LLCMK2). Dermaseptin 01 displayed antiviral effect only against Dengue virus type 2 with a 50% effective concentration of 15 μg/mL in C6/36 cells and 60 μg/mL in LLCMK2 cells. Conclusions: These data suggested that dermaseptin 01 have an in vitro antiviral action against Dengue virus type 2 but not against Herpes simplex virus type 1 or Vaccinia virus
- Subjects
ANTI-infective agents; PEPTIDES; ANTINEOPLASTIC agents; DENGUE viruses; POXVIRUS diseases
- Publication
Scientia Medica, 2013, Vol 23, Issue 1, p18
- ISSN
1806-5562
- Publication type
Article