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- Title
Organogel Nanoparticles as a New Way to Improve Oral Bioavailability of Poorly Soluble Compounds.
- Authors
Martin, Baptiste; Garrait, Ghislain; Beyssac, Eric; Goudouneche, Dominique; Perez, Emile; Franceschi, Sophie
- Abstract
Purpose: The aim of the study was to evaluate organogel nanoparticles as a lipophilic vehicle to increase the oral bioavailability of poorly soluble compounds. Efavirenz (EFV), a Biopharmaceutical Classification System (BCS) Class II, was used as drug model. Methods: Organogel nanoparticles loaded with EFV were formulated with sunflower oil, 12-hydroxystearic acid (HSA) and polyvinyl alcohol (PVA). Various parameters have been investigated in the current study such as (i) the release profile of organogel assessed by USP 4 cell flow dialysis, (ii) the impact of organogel on intestinal absorption, using Caco-2 cells as in vitro model and jejunum segments as ex vivo assay and (iii) the bioavailability of organogel following oral pharmacokinetic study. Results: 250–300 nm spherical particles with a final concentration of 4.75 mg/mL drug loading were obtained, corresponding to a thousand fold increase in EFV solubility, combined to a very high encapsulation efficiency (>99.8%). Due to rapid diffusion, drug was immediately released from the nanoparticles. The biopharmaceutical evaluation on ex vivo jejunum segments demonstrated an increased absorption of EFV from organogel nanoparticles compare to a native EFV suspension. In vitro assays combining Caco-2 cell cultures with TEM and confocal microscopy demonstrated passive diffusion, while paracellular integrity and endocytosis activity remain expelled. Oral pharmacokinetics of EFV organogel nanoparticles improve oral bioavailability (Fr: 249%) and quick absorption compared to EFV suspension. Conclusion: Organogel nanoparticles increase the bioavailability of BCS Class II drugs. The main phenomena is simply oil transfer from the gelled particles through the cell membrane.
- Subjects
BIOAVAILABILITY; NANOPARTICLES; TRANSMISSION electron microscopy; SUNFLOWER seed oil; INTESTINAL absorption; TRANSVERSE electromagnetic cells; POLYVINYL alcohol
- Publication
Pharmaceutical Research, 2020, Vol 37, Issue 6, p1
- ISSN
0724-8741
- Publication type
Article
- DOI
10.1007/s11095-020-02808-w