We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine.
- Authors
Zhu, Jiangjiang; Neto, Fausto Carnevale; Gu, Haiwei; Raftery, Daniel; Carroll, Patrick A.; Eisenman, Robert N.; Du, Jianhai
- Abstract
The balance between metabolism and biomass is very important in biological systems; however, to date there has been no quantitative method to characterize the balance. In this methodological study, we propose to use the distribution of amino acids in different domains to investigate this balance. It is well known that endogenous or exogenous amino acids in a biological system are either metabolized or incorporated into free amino acids (FAAs) or proteome amino acids (PAAs). Using glycine (Gly) as an example, we demonstrate a novel method to accurately determine the amounts of amino acids in various domains using serum, urine, and cell samples. As expected, serum and urine had very different distributions of FAA- and PAA-Gly. Using Tet21N human neuroblastoma cells, we also found that Myc(oncogene)-induced metabolic reprogramming included a higher rate of metabolizing Gly, which provides additional evidence that the metabolism of proliferating cells is adapted to facilitate producing new cells. It is therefore anticipated that our method will be very valuable for further studies of the metabolism and biomass balance that will lead to a better understanding of human cancers.
- Subjects
PROTEOMICS; GLYCINE; METABOLISM; BIOMASS; AMINO acids; NEUROBLASTOMA
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 50, p15646
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201609236