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- Title
Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses.
- Authors
Men, Yujie; Feil, Helene; VerBerkmoes, Nathan C; Shah, Manesh B; Johnson, David R; Lee, Patrick K H; West, Kimberlee A; Zinder, Stephen H; Andersen, Gary L; Alvarez-Cohen, Lisa
- Abstract
Dehalococcoides ethenogenes strain 195 (DE195) was grown in a sustainable syntrophic association with Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source. In the co- and tri-cultures, maximum dechlorination rates of DE195 were enhanced by approximately three times (11.0±0.01 μmol per day for the co-culture and 10.1±0.3 μmol per day for the tri-culture) compared with DE195 grown alone (3.8±0.1 μmol per day). Cell yield of DE195 was enhanced in the co-culture (9.0±0.5 × 107 cells per μmol Cl− released, compared with 6.8±0.9 × 107 cells per μmol Cl− released for the pure culture), whereas no further enhancement was observed in the tri-culture (7.3±1.8 × 107 cells per μmol Cl− released). The transcriptome of DE195 grown in the co-culture was analyzed using a whole-genome microarray targeting DE195, which detected 102 significantly up- or down-regulated genes compared with DE195 grown in isolation, whereas no significant transcriptomic difference was observed between co- and tri-cultures. Proteomic analysis showed that 120 proteins were differentially expressed in the co-culture compared with DE195 grown in isolation. Physiological, transcriptomic and proteomic results indicate that the robust growth of DE195 in co- and tri-cultures is because of the advantages associated with the capabilities of DVH to ferment lactate to provide H2 and acetate for growth, along with potential benefits from proton translocation, cobalamin-salvaging and amino acid biosynthesis, whereas MC in the tri-culture provided no significant additional benefits beyond those of DVH.
- Subjects
DESULFOVIBRIO vulgaris; GENETIC transcription; BACTERIAL genes; PROTEOMICS; BACTERIAL growth; BACTERIAL cultures; BACTERIAL genomes
- Publication
ISME Journal: Multidisciplinary Journal of Microbial Ecology, 2012, Vol 6, Issue 2, p410
- ISSN
1751-7362
- Publication type
Article
- DOI
10.1038/ismej.2011.111