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- Title
Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico.
- Authors
Gutiérrez-Ferman, J. L.; Villarreal-Treviño, L.; Ramírez-Aranda, J. M.; Camacho-Ortiz, A.; Ballesteros-Elizondo, M. R.; Moreno-Juárez, M. R.; Mendoza-Olazarán, S.; de la O Cavazos, M. E.; Villarreal-Pérez, J. Z.; Gómez-Govea, M. A.; Garza-González, E.
- Abstract
We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.
- Subjects
BORDETELLA pertussis; PERTUSSIS toxin; POLYMERASE chain reaction; GENOTYPES; GENOMES
- Publication
Epidemiology & Infection, 2018, Vol 146, Issue 16, p2096
- ISSN
0950-2688
- Publication type
journal article
- DOI
10.1017/S0950268818002303