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- Title
Coagulation function in fresh-frozen plasma prepared with two photochemical treatment methods: methylene blue and amotosalen.
- Authors
Osselaer, Jean-Claude; Debry, Cécile; Goffaux, Maite; Pineau, Jacqueline; Calomme, Genevieve; Dubuc, Eric; Chatelain, Bernard; Vandendaele, Marie-Claire; Hsu, Jocelyn; Rheinschmidt, Margaret; Lin, Lily
- Abstract
BACKGROUND: Pathogen inactivation of plasma intended for transfusion is now the standard of care in Belgium. Two methods for treatment of single plasma units are available: amotosalen plus ultraviolet A light and methylene blue plus visible light. This study compared the quality and stability of plasma treated with these two methods. STUDY DESIGN AND METHODS: Plasma units made from a pool of two ABO-matched fresh apheresis units were photochemically treated with either amotosalen (PCT-FFP) or methylene blue (MB-FFP). A total of 12 paired samples were evaluated. Plasma coagulation function was assessed at three time points: immediately after treatment, after 30 days of frozen storage, and an additional 24 hours at 4°C after thawing. Comparison between PCT-FFP and MB-FFP was assessed with the paired t test and a p value of less than 0.05 indicated statistical significance. RESULTS: Based on statistical analysis, mean levels of factor (F)II, FXII, FXIII, von Willebrand antigen, ADAMTS-13, D-dimers, and protein C were equivalent between PCT-FFP and MB-FFP for all three time points. PCT-FFP exhibited shorter mean prothrombin time, activated partial thromboplastin time (two time points), and thrombin time and higher mean levels of fibrinogen, FXI, and protein S than MB-FFP. Retention of FV, FVII, FVIII, FX, or von Willebrand factor:ristocetin cofactor in PCT-FFP was either equivalent to or higher than MB-FFP. MB-FFP contained higher mean levels of plasminogen, antithrombin, and plasmin inhibitor than PCT-FFP. Retention of F IX in MB-FFP was higher than PCT-FFP only after the 4°C storage after thawing. CONCLUSION: There is adequate preservation of therapeutic coagulation factor activities in both PCT-FFP and MB-FFP. The overall coagulation factor levels and stability of PCT-FFP were better preserved than MB-FFP.
- Subjects
BELGIUM; BLOOD coagulation; BLOOD plasma; PHOTOCHEMISTRY; METHYLENE blue; BLOOD transfusion; HEMAPHERESIS
- Publication
Transfusion, 2008, Vol 48, Issue 1, p108
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.1537-2995.2007.01488.x