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- Title
Genetic dissection of pre-harvest sprouting resistance in an upland rice cultivar.
- Authors
Yosuke Mizuno; Utako Yamanouchi; Tomoki Hoshino; Yasunori Nonoue; Kazufumi Nagata; Shuichi Fukuoka; Tsuyu Ando; Masahiro Yano; Kazuhiko Sugimoto
- Abstract
Seed dormancy is important in rice breeding because it confers resistance to pre-harvest sprouting (PHS). To detect quantitative trait loci (QTLs) for pre-harvest sprouting resistance, we used chromosome segment substitution lines (CSSLs) derived from a cross between the Japanese upland rice cultivar 'Owarihatamochi' and the lowland rice cultivar 'Koshihikari'. In the CSSLs, several chromosomal regions were associated with PHS resistance. Among these, the chromosome 9 segment from 'Owarihatamochi' had the greatest association with increased PHS resistance. Further QTL analysis using an advanced backcross population (BC4F2) derived from a 'Koshihikari' × 'Owarihatamochi' cross revealed two putative QTLs, here designated qSDR9.1 (Seed dormancy 9.1) and qSDR9.2, on chromosome 9. The 'Owarihatamochi' alleles of the two QTLs reduced germination. Further fine mapping revealed that qSDR9.1 and qSDR9.2 were located within 4.1-Mb and 2.3-Mb intervals (based on the 'Nipponbare' reference genome sequence) defined by the simple sequence repeat marker loci RM24039 and RM24260 and Indel_2 and RM24540, respectively. We thus identified two QTLs for PHS resistance in 'Owarihatamochi', even though resistance levels are relatively low in this cultivar. This unexpected finding suggests the advantages of using CSSLs for QTL detection.
- Subjects
RICE genetics; UPLAND rice; GERMINATION; SEED dormancy; LOCUS in plant genetics
- Publication
Breeding Science, 2018, Vol 68, Issue 2, p200
- ISSN
1344-7610
- Publication type
Article
- DOI
10.1270/jsbbs.17062