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- Title
EasyCatch, a convenient, sensitive and specific CRISPR detection system for cancer gene mutations.
- Authors
Liu, Yin; Chen, Yanling; Dang, Lu; Liu, Yixin; Huang, Shisheng; Wu, Sanyun; Ma, Peixiang; Jiang, Hongqiang; Li, Yi; Pan, Yunbao; Wei, Yongchang; Ma, Xiaodong; Liu, Ming; Ji, Quanjiang; Chi, Tian; Huang, Xingxu; Wang, Xinjie; Zhou, Fuling
- Abstract
EasyCatch accurately detected FLT3-D835Y/V/H/F mutations in clinical samples With these, AML patient samples were used for EasyCatch detection of D835Y/V/H/F mutations. EasyCatch achieves a 0.001% sensitivity in FLT3-D835Y detection With the optimized crRNAs and primers, we set out to determine the detection limit of EasyCatch for I FLT3 i -D835Y. Keywords: CRISPR detection; cancer mutation; Drug resistance; Leukemia; SNP detection EN CRISPR detection cancer mutation Drug resistance Leukemia SNP detection 1 7 7 12/06/21 20211202 NES 211202 Supplementary Information The online version contains supplementary material available at https://doi.org/10.1186/s12943-021-01456-x. Abbreviations CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Cas CRISPR associated proteins AML Acute myeloid leukemia I FLT3 i FMS-like tyrosine kinase 3 PCR Polymerase chain reaction WT Wild-type crRNA CRISPR RNA RPA Recombinase polymerase amplification RBC Red blood cell WBC White blood cell FGS First-generation sequencing NGS Next-generation sequencing Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
- Subjects
CANCER genes; EARLY detection of cancer; GENETIC mutation; CRISPRS; GENETIC variation
- Publication
Molecular Cancer, 2021, Vol 20, Issue 1, p1
- ISSN
1476-4598
- Publication type
Article
- DOI
10.1186/s12943-021-01456-x