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- Title
Experimental conditions can obscure the second high-affinity site in LeuT.
- Authors
Quick, Matthias; Shi, Lei; Zehnpfennig, Britta; Weinstein, Harel; Javitch, Jonathan A
- Abstract
Neurotransmitter:Na+ symporters (NSSs), the targets of antidepressants and psychostimulants, recapture neurotransmitters from the synapse in a Na+-dependent symport mechanism. The crystal structure of the NSS homolog LeuT from Aquifex aeolicus revealed one leucine substrate in an occluded, centrally located (S1) binding site next to two Na+ ions. Computational studies combined with binding and flux experiments identified a second substrate (S2) site and a molecular mechanism of Na+-substrate symport that depends upon the allosteric interaction of substrate molecules in the two high-affinity sites. Here we show that the S2 site, which has not yet been identified by crystallographic approaches, can be blocked during preparation of detergent-solubilized LeuT, thereby obscuring its crucial role in Na+-coupled symport. This finding points to the need for caution in selecting experimental environments in which the properties and mechanistic features of membrane proteins can be delineated.
- Subjects
NEUROTRANSMITTERS; ANTIDEPRESSANTS; STIMULANTS; AQUIFEX aeolicus; MEMBRANE proteins
- Publication
Nature Structural & Molecular Biology, 2012, Vol 19, Issue 2, p207
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2197