We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A regulator of Dscam mutually exclusive splicing fidelity.
- Authors
Olson, Sara; Blanchette, Marco; Park, Jung; Savva, Yiannis; Yeo, Gene W.; Yeakley, Joanne M.; Rio, Donald C.; Graveley, Brenton R.
- Abstract
The Down syndrome cell adhesion molecule (Dscam) gene has essential roles in neural wiring and pathogen recognition in Drosophila melanogaster. Dscam encodes 38,016 distinct isoforms via extensive alternative splicing. The 95 alternative exons in Dscam are organized into clusters that are spliced in a mutually exclusive manner. The exon 6 cluster contains 48 variable exons and uses a complex system of competing RNA structures to ensure that only one variable exon is included. Here we show that the heterogeneous nuclear ribonucleoprotein hrp36 acts specifically within, and throughout, the exon 6 cluster to prevent the inclusion of multiple exons. Moreover, hrp36 prevents serine/arginine-rich proteins from promoting the ectopic inclusion of multiple exon 6 variants. Thus, the fidelity of mutually exclusive splicing in the exon 6 cluster is governed by an intricate combination of alternative RNA structures and a globally acting splicing repressor.
- Subjects
DOWN syndrome; HUMAN chromosome abnormalities; CELL adhesion molecules; MOLECULAR genetics; DROSOPHILA melanogaster; EXONS (Genetics); SPLIT genes
- Publication
Nature Structural & Molecular Biology, 2007, Vol 14, Issue 12, p1134
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb1339