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- Title
Evaluation of tumor-infiltrating lymphocytes in osteosarcomas of the jaws: a multicenter study.
- Authors
Alves, Pollianna Muniz; de Arruda, José Alcides Almeida; Arantes, Diego Antônio Costa; Costa, Sara Ferreira Santos; Souza, Lucas Lacerda; Pontes, Hélder Antônio Rebelo; Fonseca, Felipe Paiva; Mesquita, Ricardo Alves; Nonaka, Cassiano Francisco Weege; Mendonça, Elismauro Francisco; Batista, Aline Carvalho
- Abstract
The aim of the present study was to investigate the profile of tumor-infiltrating lymphocytes (TIL) in osteosarcomas of the jaws (OSJ). A total of 21 OSJ samples were analyzed in a retrospective and cross-sectional multicenter study. Immunohistochemistry was performed to determine the recognition of TIL such as CD4+, CD8+, granzyme B+ (GrB), programmed cell death protein+ (PD-1), and cytotoxic T lymphocyte-associated antigen 4+ (CTLA-4) in intratumoral and peripheral (stromal) regions. Positivity was determined based on the percentage and density of TIL+ per square millimeter [1 = absent (< 25 cells/mm2), 2 = low (25 to 130 cells/mm2), and 3 = high (> 130 cells/mm2)]. The association of TIL density with clinicopathologic data was determined by the Mann-Whitney test (p < 0.05). OSJ were positive for CD8+ cells in 45% (n = 9) of cases, for CD4+ cells in 30% (n = 6) of cases, and for CTLA-4+ in 4.8% (n = 1) of cases, with a score of 2 (low TIL) in all cases. All cases were negative for GrB and PD-1 (score 1). No association was observed between immune infiltrate and clinicopathologic findings. OSJ showed a microenvironment with low TIL, including failure of effectiveness of the antitumor immune response (absence of GrB+ cells), and few cells exhibited immunotherapeutic targets, such as CTLA-4 and PD-1.
- Subjects
IMMUNOHISTOCHEMISTRY; LYMPHOCYTES; CD4 antigen; ORAL cancer; IMMUNOTHERAPY; ANTIGENS; JAW tumors; OSTEOSARCOMA; PROTEOLYTIC enzymes; T cells; CROSS-sectional method; RETROSPECTIVE studies
- Publication
Virchows Archiv: European Journal of Pathology, 2019, Vol 474, Issue 2, p201
- ISSN
0945-6317
- Publication type
journal article
- DOI
10.1007/s00428-018-2499-6