We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Poor prognosis in Epstein-Barr virus-negative gastric cancer with lymphoid stroma is associated with immune phenotype.
- Authors
Cho, Charles J.; Kang, Hyo Jeong; Ryu, Yeon-Mi; Park, Young Soo; Jeong, Hui Jeong; Park, Young-Mi; Lim, Hyun; Lee, Jeong Hoon; Song, Ho June; Jung, Hwoon-Yong; Kim, Sang-Yeob; Myung, Seung-Jae
- Abstract
Background: Gastric cancer with lymphoid stroma (GCLS) is pathologically characterized by poorly developed tubular structures with a prominent lymphocytic infiltration. Its clinical and prognostic features differ in patients positive and negative for Epstein-Barr virus (EBV) infection. This study analyzed the expression of programmed cell death-1 (PD-1), programmed cell death ligand-1 (PD-L1), and the density of tumor-infiltrating lymphocytes (TILs) including CD3+ and CD8+ T cells, as well as their prognostic significance in patients with GCLS.Methods: The study included 58 patients with GCLS (29 EBV+ and 29 EBV−) who underwent curative resection. Expression of CD3, CD8, PD-1, and PD-L1 in tumor cells and TILs was analyzed using a quantitative multispectral imaging system (Opal™), with these results validated by immuno-histochemical assays for PD-L1 on whole slide sections.Results: The proportion of tumors overexpressing PD-L1 (31.0 vs. 0%, P = 0.002), TIL density (4548 vs. 2631/mm2, P < 0.001), and intra-tumoral CD8+ T-cell density (2650 vs. 1060/mm2, P < 0.001) were significantly higher in EBV+ than in EBV− GCLS. In addition, CD8+/CD3+ T-cell ratio was higher in EBV+ than in EBV− GCLS (55.3 vs. 35.8%, P < 0.001). Lower TIL density, defined as < 1350/mm2, was a significant negative factor of survival.Conclusions: Despite histopathological similarity, quantitative multispectral imaging revealed differences in the tumor immune micro-environment between EBV+ and EBV− GCLS, indicating that the underlying pathogenesis differs in these two disease entities. TIL density may be a prognostic marker in patients with GCLS.
- Subjects
ABDOMINAL cancer; PHENOTYPES; EPSTEIN-Barr virus; HUMAN experimentation; IMAGING systems
- Publication
Gastric Cancer, 2018, Vol 21, Issue 6, p925
- ISSN
1436-3291
- Publication type
Article
- DOI
10.1007/s10120-018-0820-3