We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Different mechanisms of resistance modulate sulfite tolerance in wine yeasts.
- Authors
Nadai, Chiara; Treu, Laura; Campanaro, Stefano; Giacomini, Alessio; Corich, Viviana
- Abstract
From a technological point of view, yeast resistance to sulfite is of great interest and represents an important technological character for winemaking. Several mechanisms are involved, and strain-dependent strategies to obtain SO resistance can deeply influence wine quality, although this choice is less relevant in determining the technological performance of the strain during fermentation. In this study, to better understand the strain-specific mechanisms of resistance, 11 Saccharomyces cerevisiae strains, whose genomes have been previously sequenced, were selected. Their attitude towards sulfites, in terms of resistance and production, was evaluated, and RNA-sequencing of four selected strains was performed during fermentation process in synthetic grape must in the presence of SO. Results demonstrated that at molecular level, the physical effect of SO triggered multiple stress responses in the cell and high tolerance to general enological stressing condition increased SO resistance. Adaptation mechanism due to high basal gene expression level rather than specific gene induction in the presence of sulfite seemed to be responsible in modulating strain resistance. This mechanism involved higher basal gene expression level of specific cell wall proteins, enzymes for lipid biosynthesis, and enzymes directly involved in SO assimilation pathway and efflux.
- Subjects
SULFITES; WINE microbiology; YEAST; FERMENTATION; FUNGAL gene expression
- Publication
Applied Microbiology & Biotechnology, 2016, Vol 100, Issue 2, p797
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-015-7169-x