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- Title
The ameliorating effect of silymarin against vancomycininduced apoptosis and inflammation in rat liver.
- Authors
GÜZEL, Sevda; UÇKUN ŞAHİNOĞULLARI, Zuhal; CANACANKATAN, Necmiye; ANTMEN, Şerife Efsun; KİBAR, Deniz; BAYRAK, Gülsen
- Abstract
Silymarin (SL), a flavonolignan complex isolated from seeds of Silybum marianum (Asteraceae), is known for its hepatoprotective, anti-apoptotic, anti-inflammatory, and antioxidant activities. A glycopeptide antibiotic, Vancomycin (VA) which is used for the treatment of serious infections caused by multi-resistant Gram-positive microorganisms has been clinically used for a long time. The aim of the present study was to evaluate potential therapeutic efficiency of SL against VA-induced apoptosis and inflammation using apoptotic (caspase-3, -8, and, -9 enzyme activities) and inflammatory (Tumor necrosis factor-alpha (TNF-a)) markers, and histopathological examinations in rat liver. A total of 49 male Wistar albino rats was divided into 7 groups including control (saline, intraperitoneally (i.p.)), Dimethyl sulfoxide (i.p.), VA (400 mg/kg/day, i.p.), SL100 (100 mg/kg/day, i.p.), VA+SL50 (50 mg/kg/day, i.p.), VA+SL100 (100 mg/kg/day, i.p.), and VA+SL200 (200 mg/kg/day, i.p.). SL was administered once a daily for 8 days. One day after the first treatment of SL, VA administration was started and continued for 7 days. Hepatic TNF-a levels were evaluated by ELISA and hepatic caspase activities were evaluated according to the colorimetric method. Significantly increased caspase activities were determined in VA group compared to control group (P<0.05). However, significantly reduced caspase activities were determined in VA+SL200 group when compared to VA group (P<0.05). TNF-a level in VA group was found to be statistically higher than control group (P<0.05). TNF-a levels of SL100, VA+SL (50, 100, and 200) groups were significantly lower than VA group (P<0.05). VA+SL200 group was found to be the most effective group according to reducing caspase activities and TNF-a level.
- Subjects
HEPATITIS; GLYCOPEPTIDE antibiotics; DRUG resistance in microorganisms; DIMETHYL sulfoxide; MILK thistle; APOPTOSIS; OXAZOLIDINONES
- Publication
Journal of Research in Pharmacy, 2019, Vol 23, Issue 4, p719
- ISSN
2630-6344
- Publication type
Article
- DOI
10.12991/jrp.2019.181