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- Title
Niches and routes of transmission of <italic>Xanthomonas citri</italic> pv. <italic>fuscans</italic> to bean seeds.
- Authors
Darrasse, Armelle; Barret, Matthieu; Cesbron, Sophie; Compant, Stéphane; Jacques, Marie-Agnès
- Abstract
Aims: Seeds are vectors of a diversified microbiota including plant pathogens. To better understand transmission of common bacterial blight (CBB) agents to bean seeds, we analyzed the role of non-pathogenic xanthomonads on seed transmission efficiency and investigated the location of <italic>Xanthomonas citri</italic> pv. <italic>fuscans</italic> (<italic>Xcf</italic>) into seeds and plantlets.Methods: Competition between CBB and NP strains was initially assessed in vitro and then extended <italic>in planta</italic> to monitor the impact of co-inoculation on <italic>Xcf</italic> seed transmission. Moreover, location of <italic>Xcf</italic> strains in seeds and seedlings was visualized using a combination of <italic>gfp-</italic>tagged strain and DOPE-FISH/CSLM.Results: Whereas CBB agent growth was inhibited in vitro by some seed-borne non-pathogenic xanthomonads strains, these strains did not transmit efficiently to seed through floral pathway and did not affect <italic>Xcf</italic> seed transmission. <italic>Xcf</italic> cells were observed entering seed through vascular elements and parenchyma of funiculus, but also micropyle and testa. <italic>Xcf</italic> cells were observed, moreover, among other bacteria on radicle surfaces, especially tip, in cotyledons, and plumules.Conclusions: CBB agents are more efficient than non-pathogenic xanthomonads in using the floral route to colonize seeds. CBB agents are located within different niches in the seed tissues up to the embryonic axis.
- Subjects
SEED microbiology; BLIGHT diseases (Botany); XANTHOMONAS campestris; ECOLOGICAL niche; CONFOCAL microscopy
- Publication
Plant & Soil, 2018, Vol 422, Issue 1/2, p115
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-017-3329-3