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- Title
Influence of Cycloheximide and Actinomycin D on Initiation and Early Development Adventitious Roots.
- Authors
Oppenoorth, J. M.
- Abstract
From leaf cuttings of the bean Phaseolus vulgaris L. adventitious roots form on the petiole. This root formation is stimulated by treatment with auxin. Simultaneous or subsequent application of cycloheximide irreversibly inhibited dedifferentiation, so that root production was completely prevented. The effects of actinomycin D application depended upon the stage of development of the root primordium. Cells in the first stage of dedifferentiation were extremely sensitive. When actinomycin D was applied later than 6 h after cutting, its inhibiting effect gradually diminished. It is concluded that an actinomycin D-sensitive process occur- ring early in dedifferentiation is crucial for root initiation. A second, less actinomycin D-sensitive process occurring later in dedifferentiation is required for the further development of the root primordium. During the initiation and development of the root primordium protein synthesis is required.
- Subjects
ACTINOMYCIN; ANTINEOPLASTIC antibiotics; INITIATION rites; CELLS; PHYSIOLOGY; PROTOPLASM
- Publication
Physiologia Plantarum, 1979, Vol 47, Issue 2, p134
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1979.tb03204.x