We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Low Maternal Microbiota Sharing across Gut, Breast Milk and Vagina, as Revealed by 16S rRNA Gene and Reduced Metagenomic Sequencing.
- Authors
Avershina, Ekaterina; Angell, Inga Leena; Simpson, Melanie; Storrø, Ola; Øien, Torbjørn; Johnsen, Roar; Rudi, Knut
- Abstract
The maternal microbiota plays an important role in infant gut colonization. In this work we have investigated which bacterial species are shared across the breast milk, vaginal and stool microbiotas of 109 women shortly before and after giving birth using 16S rRNA gene sequencing and a novel reduced metagenomic sequencing (RMS) approach in a subgroup of 16 women. All the species predicted by the 16S rRNA gene sequencing were also detected by RMS analysis and there was good correspondence between their relative abundances estimated by both approaches. Both approaches also demonstrate a low level of maternal microbiota sharing across the population and RMS analysis identified only two species common to most women and in all sample types (<italic>Bifidobacterium longum</italic> and <italic>Enterococcus faecalis</italic>). Breast milk was the only sample type that had significantly higher intra- than inter- individual similarity towards both vaginal and stool samples. We also searched our RMS dataset against an in silico generated reference database derived from bacterial isolates in the Human Microbiome Project. The use of this reference-based search enabled further separation of <italic>Bifidobacterium longum</italic> into <italic>Bifidobacterium longum</italic> ssp. <italic>longum</italic> and <italic>Bifidobacterium longum</italic> ssp. <italic>infantis</italic>. We also detected the <italic>Lactobacillus rhamnosus</italic> GG strain, which was used as a probiotic supplement by some women, demonstrating the potential of RMS approach for deeper taxonomic delineation and estimation.
- Subjects
METAGENOMICS; HUMAN microbiota; ENTEROCOCCUS faecalis; BREAST milk; RIBOSOMAL RNA
- Publication
Genes, 2018, Vol 9, Issue 5, p231
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes9050231