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- Title
Heat shock protein expression in oral lichen planus.
- Authors
Sugerman, P. B.; Savage, N. W.; Xu, L. J.; Walsh, L. J.; Seymour, G. J.
- Abstract
To assess the potential role of heat shock protein (HSP) in the pathogenesis of oral lichen planus (OLP), sections of OLP, normal oral mucosa, non-specific oral ulceration (NSOU) and dysplastic OLP were assessed for HSP expression using avidin-biotin complex immunohistochemistry with an anti-HSP 70 polyclonal antibody. There were statistically significant differences in both the vertical and horizontal staining distribution when other groups were compared with the OLP group (p<0.01). Using microdensitometry, the mean staining intensity in OLP, dysplastic OLP and NSOU was elevated in comparison with normal oral mucosa (p<0.001). In a standard tritiated thymidine uptake assay, lymphocytes extracted from nine OLP lesions demonstrated significant proliferation when stimulated with purified protein derivative (PPD), of which HSP is a major constituent, with stimulation indices ranging from 2 to 132. These results are consistent with the hypothesis that, in OLP patients, diverse exogenous agenst may cause upregulated expression of HSP by oral mucosal keratinocytes. A reaction of cytotoxic T lymphocytes to these activated keratinocytes may then result in the tissue destruction which is characteristic of OLP lesions.
- Subjects
HEAT shock proteins; IMMUNOHISTOCHEMISTRY; LICHEN planus; PROTEINS; ANALYSIS of variance; BIOCHEMISTRY; COMPARATIVE studies; DENSITOMETRY; IMMUNOENZYME technique; KERATINOCYTES; PHENOMENOLOGY; RESEARCH methodology; MEDICAL cooperation; ORAL mucosa; REGRESSION analysis; RESEARCH; T cells; EVALUATION research; CASE-control method; ORAL lichen planus
- Publication
Journal of Oral Pathology & Medicine, 1995, Vol 24, Issue 1, p1
- ISSN
0904-2512
- Publication type
journal article
- DOI
10.1111/j.1600-0714.1995.tb01121.x