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- Title
The clinical characteristics of pediatric patients infected by SARS-CoV-2 Omicron variant and whole viral genome sequencing analysis.
- Authors
Tsang, Hin Fung; Yu, Allen Chi Shing; Yim, Aldrin Kay Yuen; Jin, Nana; Wu, Yu On; Cheng, Hennie Yuk Lin; Cheung, WL; Leung, Wai Ming Stanley; Lam, Ka Wai; Hung, Tin Nok; Chan, Loiston; Chiou, Jiachi; Pei, Xiao Meng; Lee, On Ying Angela; Cho, William Chi Shing; Wong, Sze Chuen Cesar
- Abstract
Pediatric population was generally less affected clinically by SARS-CoV-2 infection. Few pediatric cases of COVID-19 have been reported compared to those reported in infected adults. However, a rapid increase in the hospitalization rate of SARS-CoV-2 infected pediatric patients was observed during Omicron variant dominated COVID-19 outbreak. In this study, we analyzed the B.1.1.529 (Omicron) genome sequences collected from pediatric patients by whole viral genome amplicon sequencing using Illumina next generation sequencing platform, followed by phylogenetic analysis. The demographic, epidemiologic and clinical data of these pediatric patients are also reported in this study. Fever, cough, running nose, sore throat and vomiting were the more commonly reported symptoms in children infected by Omicron variant. A novel frameshift mutation was found in the ORF1b region (NSP12) of the genome of Omicron variant. Seven mutations were identified in the target regions of the WHO listed SARS-CoV-2 primers and probes. On protein level, eighty-three amino acid substitutions and fifteen amino acid deletions were identified. Our results indicate that asymptomatic infection and transmission among children infected by Omicron subvariants BA.2.2 and BA.2.10.1 are not common. Omicron may have different pathogenesis in pediatric population.
- Subjects
SARS-CoV-2 Omicron variant; WHOLE genome sequencing; FRAMESHIFT mutation; CHILD patients; NUCLEOTIDE sequencing; SEQUENCE analysis; VIRAL genomes
- Publication
PLoS ONE, 2023, Vol 17, Issue 3, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0282389