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- Title
Disruption of Phospholipase Cδ4 Gene Modulates the Liver Regeneration in Cooperation with Nuclear Protein Kinase C.
- Authors
Akutagawa, Atsushi; Fukami, Kiyoko; Banno, Yoshiko; Takenawa, Tadaomi; Kannagi, Reiji; Yokoyama, Yukihiro; Oda, Koji; Nagino, Masato; Nimura, Yuji; Yoshida, Shonen; Tamiya-Koizumi, Keiko
- Abstract
Phospholipase Cδ4 (PLC δ4) gene has been cloned from the cDNA library of regenerating rat liver. Using PLC δ4 gene–disrupted mice (PLC δ4−/−), we studied a role of PLC δ4 during liver regeneration after partial hepatectomy (PH). In PLC δ4−/−, liver regeneration occurred in an apparently normal way. However, BrdU-indices indicated that PLC δ4 gene disruption delayed the onset of DNA synthesis by 2 h. Noticeably, the BrdU-indices in PLC δ4+/+ remained rather constant throughout S phase, 25–35%, whereas in PLC δ4−/−, it fluctuated drastically from 25% at 34 h to 65% at late S, 42 h after PH. This fact showed that PLC δ4 gene disruption caused a higher synchronization of cell proliferation. The mRNA for PLC δ4 in PLC δ4+/+ appeared at late G1, and the expression continued throughout S phase. PLC activity increased transiently in chromatin at the late G1 and S phases in only PLC δ4+/+, but not in PLC δ4−/−. The specific increases in PLC activity well correlated with the transient increases of protein kinase C (PKC) α in chromatin of PLC δ4+/+. PKC ɛ also increased transiently in chromatin from PLC δ4+/+ at late S. It is concluded that PLC δ4 regulates the liver regeneration in cooperation with nuclear PKC α and ɛ.
- Subjects
PHOSPHOLIPASE C; LIVER regeneration; PROTEIN kinase C; LABORATORY mice; HEPATECTOMY
- Publication
Journal of Biochemistry, 2006, Vol 140, Issue 5, p619
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvj194