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- Title
Study of the polymorphisms of cyclooxygenase-2 (-765G>C) and 5-lipoxygenase (1708G>A) in patients with colorectal cancer.
- Authors
MARQUES PIMENTA, CÉLIA APARECIDA; MOREIRA LATINI, FLAVIA ROCHE; MIRANDA DE LIMA, JACQUELINE; DONIZETTI DA SILVA, TIAGO; VITOR FELIPE, ALEDSON; DE LIMA PAZINE, VANESSA MARIA; MANOUKIAN FORONES, NORA
- Abstract
Colorectal cancer (CRC) is the fourth most common cause of cancer-related mortality worldwide. Genetic alterations have been associated with an increased risk of cancer and greater tumor aggressiveness. Cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) genes are important in cell cycle regulation, tumor growth and prostaglandin synthesis. The aim of the present study was to investigate the association between polymorphisms in the COX-2 and 5-LOX genes and the risk of CRC. A case-control study was conducted in patients with CRC matched for gender and age to a control group. DNA was extracted from peripheral leukocytes, and the polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. A specific questionnaire was applied to evaluate smoking, excessive alcohol consumption, physical activity, non-steroidal anti-inflammatory drug use and meat, fiber and fat intake. A total of 185 patients with CRC and 146 controls were studied. The heterozygous GC genotype of the COX-2 gene polymorphism was the most common in the two groups (60.0% in CRC patients and 52.7% in controls). The CC genotype was associated with an increased risk of CRC (odds ratio, 3.63; 95% confidence interval, 1.31-10.1; P=0.013). The homozygous wild-type genotype of the 5-LOX gene polymorphism was detected in 72.4% of the CRC patients and in 71.2% of the control subjects. The homozygous mutant genotype (CC) of the COX-2 gene is an independent risk factor for CRC. No association was found between 5-LOX genotypes and CRC.
- Subjects
GENETIC polymorphism research; CYCLOOXYGENASE genetics; LIPOXYGENASE genetics; GENETICS of colon cancer; TUMOR growth; PROSTAGLANDIN synthesis; DNA polymerase genetics
- Publication
Oncology Letters, 2014, Vol 7, Issue 2, p513
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2013.1732