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- Title
Low-intensity pulsed ultrasound inhibits lipopolysaccharide-induced IL-6 and RANKL expression in osteoblasts.
- Authors
Mayu Nagao; Natsuko Tanabe; Soichiro Manaka; Tadahiro Takayama; Takayuki Kawato; Go Torigoe; Jumpei Sekino; Naoya Tsukune; Manami Ozaki; Masao Maeno; Naoto Suzuki; Shuichi Sato; Nagao, Mayu; Tanabe, Natsuko; Manaka, Soichiro; Takayama, Tadahiro; Kawato, Takayuki; Torigoe, Go; Sekino, Jumpei; Tsukune, Naoya
- Abstract
Periodontal disease is caused by inflammation induced by Porphyromonas gingivalis (P.g.) lipopolysaccharide (LPS) and involves expression of proinflammatory cytokines such as interleukin (IL)-1, IL-6, tumor necrosis factor-α, and receptor activator of nuclear factor kappa B ligand (RANKL), which are implicated in bone resorption. Low-intensity pulsed ultrasound (LIPUS) is commonly used in the treatment of bone fracture. However, the mechanisms by which LIPUS inhibits LPS-induced inflammatory cytokines are poorly understood. Therefore, we investigated the effects of LIPUS on LPS-induced expression of the proinflammatory cytokines IL-6 and RANKL. MC3T3-E1 cells were incubated in the presence or absence of P.g. LPS and then stimulated with LIPUS for 30 min/day for a maximum of 14 days. LPS increased mRNA and protein expressions of IL-6 and RANKL on day 14. In addition, mRNA expression of COX-2 LPS was higher after 3 and 7 days of LIPUS treatment. PGE2 was induced by LPS after 7 and 14 days of culture. LIPUS suppressed all stimulatory effects of LPS. These results suggest that LIPUS inhibits LPS-induced expression of inflammation cytokines by suppressing PGE2 production and might thus have potential applications in the treatment of periodontitis.
- Subjects
LIPOPOLYSACCHARIDES; OSTEOBLASTS; INTERLEUKINS; CYTOKINES; PROTEIN expression; OSTEOBLAST metabolism; ANIMALS; CELLS; ENZYME-linked immunosorbent assay; MEMBRANE proteins; MICE; POLYMERASE chain reaction; ULTRASONICS
- Publication
Journal of Oral Science, 2017, Vol 59, Issue 2, p303
- ISSN
1343-4934
- Publication type
journal article
- DOI
10.2334/josnusd.16-0624