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- Title
Expression of Plasmodium falciparum G6PD-6PGL in laboratory parasites and in patient isolates in G6PD-deficient and normal Nigerian children.
- Authors
Sodeinde, Olugbemiro; Clarke, Julia L.; Vulliamy, Tom J.; Luzzatto, Lucio; Mason, Philip J.
- Abstract
Summary. As the production of NADPH in the pentose phosphate pathway is the main antioxidant defence mechanism available to the Plasmodium falciparum , we have studied the expression of P. falciparum glucose 6-phosphate dehydrogenase-6-phosphogluconolactonase (PfG6PD-6PGL) in G6PD-deficient and normal erythrocyte host cells. Both erythrocytes infected in vitro with a laboratory isolate and erythrocytes from natural human infections were used. Total RNA was prepared from parasites collected from five G6PD-deficient and nine G6PD-normal children in Ibadan, Nigeria, selected after screening 189 rural schoolchildren and 68 clinical malaria patients, and was subjected to Northern blot analysis. The probe was a cDNA fragment of the G6PD domain of the PfG6PD-6PGL gene, with an internal control probe of P. falciparum 18S ribosomal RNA. Quantification was performed using a phosphoimager. Relative to internal control, the abundance of PfG6PD-6PGL mRNA (mean ± standard deviation) was lower in parasites from G6PD-deficient children (0·29 ± 0·27) than in G6PD-normal control subjects (0·74 ± 0·26) (P = 0·014, Mann–Whitney U -test). Although confirmation in a larger study is required, our results suggest a lower relative abundance of PfG6PD-6PGL, and presumably antioxidant activity, in malaria parasites from G6PD-deficient hosts, thus extending the current knowledge of the mechanism of G6PD-deficiency related host protection.
- Subjects
NIGERIA; PLASMODIUM falciparum; ERYTHROCYTES; PEDIATRIC hematology
- Publication
British Journal of Haematology, 2003, Vol 122, Issue 4, p662
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2003.04397.x