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- Title
Radiographic and Micro-Computed Tomographic Imaging of Lipopolysaccharide-Mediated Bone Resorption.
- Authors
Nason, Robert; Lee, Dong H.; Jung, Jae Y.; Chole, Richard A.
- Abstract
Objectives: Chronic otitis media and cholesteatomas cause hearing loss as a result of bony erosion. This bone resorption is know to be more aggressive when cholesteatomas become infected. The most common organism isolated from both diseases is the gram-negative bacterium Pseudomonas aeruginosa. Lipopolysaceharide (LPS), a major virulence actor found in the gram-negative bacterial cell wall, is well known to incite inflammatory bone resorption The mechanisms underlying this process. however, are poorly understood. In this study, we developed a mouse model of calvarial osteolysis in which resorption was reliably imaged by plain radiography and micro-computed tomography (micro-CT). Methods: A murine calvarial model was developed to study hone resorption induced by P aeruginosa LPS. Calvariate from wild-type and knockout mice used in this model were imaged by plain radiography and micro-CT. Results: A high degree of correlation between plain radiography and micro-CT was identified (R2= 0.8554). Further- more, maximal LPS-induced bone resorption required functioning toll-like receptor (TLR) 2, TLR4. and myeloid differentiation factor 88 (MyD88). Conclusions; We have developed a Successful model of inflammatory osteolysis in which plain radiography can reliably delineate induced bone resorption. In vivo, we have shown that P acruginosa LPS signals via TLR2. as well as TLR4 through MyD88.
- Subjects
BONE resorption; OSTEORADIOGRAPHY; TOMOGRAPHY; OTITIS; CHOLESTEATOMA; DEAFNESS; GRAM-negative bacteria; PSEUDOMONAS aeruginosa
- Publication
Annals of Otology, Rhinology & Laryngology, 2009, Vol 118, Issue 5, p391
- ISSN
0003-4894
- Publication type
Article
- DOI
10.1177/000348940911800512