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- Title
The Antibacterial Mechanism of Terpinen-4-ol Against Streptococcus agalactiae.
- Authors
Zhang, Yuetian; Feng, Ruizhang; Li, Lixia; Zhou, Xun; Li, Zhengwen; Jia, Renyong; Song, Xu; Zou, Yuanfeng; Yin, Lizi; He, Changliang; Liang, Xiaoxia; Zhou, Wanhai; Wei, Qin; Du, Yonghua; Yan, Kuan; Wu, Zili; Yin, Zhongqiong
- Abstract
Streptococcus agalactiae, a highly contagious mastitis pathogen, caused huge economic losses; meanwhile, repeated use of antibiotics results in the emergence of serious antibiotic residues and drug resistance. Therefore, it is in great need to develop ecologically sustainable antimicrobial agents. In the study, the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and action mechanism of terpinen-4-ol against S. agalactiae was investigated to evaluate antibacterial activity of terpinen-4-ol. Results showed the MIC and MBC of terpinen-4-ol were 98 and 196 µg/mL, respectively. Time-kill curves displayed that the antibacterial activity of terpinen-4-ol was in a concentration-dependent manner. Transmission electron micrographs showed that the cell membrane and wall of S. agalactiae were damaged, and plasmolysis and chromatins were inconspicuous. Release of Ca2+ and Mg2+ proved that terpinen-4-ol could increase cell membrane permeability. And the release of lactate dehydrogenase (LDH) suggested that cell wall was destroyed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 4′,6-diamidino-2-phenylindole (DAPI) staining results showed that terpinen-4-ol could affect the synthesis of protein and DNA. These results suggested that terpinen-4-ol might be used as candidate for treating S. agalactiae infection.
- Subjects
ANTIBACTERIAL agents; STREPTOCOCCUS agalactiae; INHIBITORY Concentration 50; LACTATE dehydrogenase; SODIUM dodecyl sulfate
- Publication
Current Microbiology, 2018, Vol 75, Issue 9, p1214
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-018-1512-2