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- Title
Ku80 Gene is Related to Non-Homologous End-Joining and Genome Stability in Aspergillus niger.
- Authors
Jinxiang Zhang; Zhihui Mao; Wei Xue; Ying Li; Guomin Tang; Aoquan Wang; Yingjiu Zhang; Huaming Wang
- Abstract
In this study, the ku70 and ku80 homologs from the Aspergillus niger genome were identified and their function was analyzed using targeted mutagenesis. The role of the ku80 gene in non-homologous end-joining (NHEJ) was investigated by calculating the frequency of homologous recombination. The transformation test verified that the frequency of homologous recombination significantly increased, from 1.78 to 65.6% in ku80 single deletion strains and to 100% in ku70/ ku80 double deletion strains. These results suggest that the ku80 gene is important for non-homologous end-joining. Although the morphology of the ku deletion strains colonies was similar to that of the wildtype strain, mutants were more sensitive to the mutagen phleomycin. Furthermore, the purified ku80 deletion strain produced some sectored colonies on hygromycin B -containing plates. This result suggests that the ku80 gene deletion leads to genomic instability in A. niger.
- Subjects
HOMOLOGY (Biology); COMPARATIVE anatomy; GENOMES; ASPERGILLUS; MUTAGENESIS; BIOLOGICAL research
- Publication
Current Microbiology, 2011, Vol 62, Issue 4, p1342
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-010-9853-5