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- Title
New Insights in Trichoderma harzianum Antagonism of Fungal Plant Pathogens by Secreted Protein Analysis.
- Authors
Monteiro, Valdirene Neves; Nascimento Silva, Roberto do; Steindorff, Andrei Stecca; Costa, Fabio Teles; Noronha, Eliane Ferreira; Ricart, Carlos André Ornelas; Sousa, Marcelo Valle de; Vainstein, Marilene Henning; Ulhoa, Cirano José
- Abstract
Trichoderma harzianum ALL42 were capable of overgrowing and degrading Rhizoctonia solani and Macrophomina phaseolina mycelia, coiling around the hyphae with formation of apressoria and hook-like structures. Hyphae of T. harzianum ALL42 did not show any coiling around Fusarium sp. hyphae suggesting that mycoparasitism may be different among the plant pathogens. In this study, a secretome analysis was used to identify some extracellular proteins secreted by T. harzianum ALL42 after growth on cell wall of M. phaseolina, Fusarium sp., and R. solani. The secreted proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. A total of 60 T. harzianum ALL42 secreted proteins excised from the gel were analyzed from the three growth conditions. While seven cell wall-induced proteins were identified, more than 53 proteins spots remain unidentified, indicating that these proteins are either novel proteins or proteins that have not yet been sequenced. Endochitinase, β-glucosidase, α-mannosidase, acid phosphatase, α-1,3-glucanase, and proteases were identified in the gel and also detected in the supernatant of culture.
- Subjects
TRICHODERMA; RHIZOCTONIA solani; MACROPHOMINA phaseolina; HYPHAE of fungi; FUSARIUM genetics; MYCOPARASITISM; BIOLOGICAL control of plant diseases; ELECTROPHORESIS
- Publication
Current Microbiology, 2010, Vol 61, Issue 4, p298
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-010-9611-8