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- Title
Experimental evidence for the lack of sensitivity of in vivo faecal egg count reduction testing for the detection of early development of benzimidazole resistance.
- Authors
Königová, Alžbeta; Urda Dolinská, Michaela; Babják, Michal; von Samson-Himmelstjerna, Georg; Komáromyová, Michaela; Várady, Marián
- Abstract
The objective of this study was to compare the results of an in vitro egg hatch test (EHT), micro-agar larval development test (MALDT) and in vivo faecal egg count reduction test (FECRT) between worm strains obtained from goats and sheep identically infected with the gastrointestinal parasitic nematode Haemonchus contortus. Results from the in vivo and in vitro tests were compared with benzimidazole (BZ)-resistance-associated β-tubulin allele frequencies determined using Pyrosequencing™. BZ resistance was not detected by the in vivo FECRT, where reductions of > 99% for both the resistant and the susceptible H. contortus strains were detected in both species. Discriminating doses in EHT and MALDT for the resistant strain indicated a low level (approx. 25%) of resistant individuals. Genotyping indicated that the susceptible strain had 10% BZ-resistant β-tubulin codon 200 alleles and the resistant strain had 26% respective resistant alleles. The in vitro tests and allele-frequency distribution suggested low levels of resistance in both strains; however, the FECRT did not support the evidence of resistant individuals of either strain in either species, suggesting a potential underestimation of low-level resistance in sheep and goats when employing this test.
- Subjects
HAEMONCHUS contortus; SHEEP breeding; GOAT diseases; ANTHELMINTICS; EGG incubation; GENE frequency; EGGS; IN vivo studies
- Publication
Parasitology Research, 2021, Vol 120, Issue 1, p153
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-020-06965-0