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- Title
Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer.
- Authors
Ralser, Damian J.; Condic, Mateja; Klümper, Niklas; Ellinger, Jörg; Staerk, Christian; Egger, Eva K.; Kristiansen, Glen; Mustea, Alexander; Thiesler, Thore
- Abstract
Purpose: N6-methyladenosine (m6A) is the most frequent type of messenger RNA (mRNA) modification and is implicated in diverse physiological processes. The procedure of m6A RNA modification is regulated by a dynamic interaction of writers (METTL3, METTL4, METTL14, WTAP, KIAA1429), erasers (FTO, ALKBH5), and readers (HNRNPA2B1, HNRNPC, YTHDC1, YTHDC1, YTHDF1-3). In the oncological context, alterations in m6A were identified to be critically involved in tumorigenesis, proliferation, angiogenesis, and drug resistance across diverse cancer entities including endometrial cancer (EC). Methods: In this study, we comprehensively examined the protein expression of m6A writers, readers and erasers by immunohistochemical staining in a cohort of N = 65 EC patients. Protein expression data were analyzed with regard to clinical outcomes. Results: We identified enhanced protein expression levels of METTL3, METTL14, FTO, HNRNPA2B1, and HNRNPC, respectively to be of prognostic value and linked to a shortened overall survival in EC. Conclusion: Overall, our study points toward dysregulated m6A modification in EC and its possibility to serve as a promising prognostic biomarker.
- Subjects
ENDOMETRIAL cancer; ADENOSINES; IMMUNOHISTOCHEMISTRY; RNA modification &; restriction; IMMUNOSTAINING
- Publication
Journal of Cancer Research & Clinical Oncology, 2023, Vol 149, Issue 6, p2417
- ISSN
0171-5216
- Publication type
Article
- DOI
10.1007/s00432-022-04083-1