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- Title
Production of transgenic pineapple (Ananas cosmos (L.) Merr.) plants via adventitious bud regeneration.
- Authors
M.-L. Wang; G. Uruu; L. Xiong; X. He; C. Nagai; K. Cheah; J. Hu; B. Sipes; H. Atkinson; P. Moore; K. Rohrbach; R. Paull
- Abstract
Abstract  A new protocol for the production of transgenic pineapple plants was developed. Adventitious buds were induced directly from Agrobacterium-infected leaf bases and stem discs of in vitro plants, bypassing the establishment of callus cultures. Non-chimeric transgenic plants were obtained by multiple subculturing of primary transformants under increasing levels of selection. A total of 42 independent transgenic lines were produced from two cultivars with two different constructs: one containing a modified rice cystatin gene (Oc-IÎD86) and the other with the anti-sense gene to pineapple aminocyclopropane synthase (ACS). GUS histochemical staining provided the first evidence of the non-chimeric nature of the transformed plants. Their non-chimeric nature was further demonstrated by PCR analyses of the DNA extracted from individual leaves of a primary transformed plant and also from multiple plants propagated from a single transformation event. Southern hybridization confirmed random integration patterns of transgenes in the independent lines. For the Oc-IÎD86 gene, the expression at the mRNA level was detected via RT-PCR and its translation was detected by protein blot. Agronomic evaluation and bioassays of the transgenic plants will further validate the utility of this new tool for pineapple improvement.
- Subjects
PLANT hybridization; PLANT breeding; BOTANY; SOUTHERN blot
- Publication
In Vitro Cellular & Developmental Biology Plant, 2009, Vol 45, Issue 2, p112
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-009-9208-8