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- Title
Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach.
- Authors
Hordijk, Joost; Mevius, Dik J.; Kant, Arie; Bos, Marian E. H.; Graveland, Haitske; Bosman, Angela B.; Hartskeerl, Cedric M.; Heederik, Dick J. J.; Wagenaar, Jaap A.
- Abstract
Objectives To assess the within-farm dynamics of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli in veal calves. Methods Three veal-calf fattening farms were screened. Faecal samples from all calves within a compartment (109–150 per farm) were taken upon arrival on the farm (T0) and after 3, 6, 8 and 10 weeks (T3–T10). ESBL/AmpC genes were characterized by PCR and sequencing. Plasmids were characterized by transformation, PCR-based replicon typing and plasmid multilocus sequence typing (MLST). E. coli genotypes were analysed by MLST. Results At T0 the prevalence of ESBL/AmpC-producing E. coli ranged from 18% to 26%. These were predominantly isolates carrying blaCTX-M-1 and blaCTX-M-15 genes, located on various plasmids and E. coli sequence types (STs). Farm 1 was negative for ESBL/AmpC-producing E. coli after T0. Farm 2 showed an increase up to 37% at T3, which subsequently decreased gradually to 0% at T10. The presence from T3 to T10 on farm 2 was mainly caused by the clonal spread of a multiresistant E. coli ST57 harbouring blaCTX-M-14 on an IncF F2:A-:B- plasmid. Farm 3 showed a gradual decrease in prevalence to 1.4% at T10, with a relative increase of the identical clonal variant as shown for farm 2. A second clonal variant found in farm 3 was a multiresistant E. coli ST10 harbouring blaCTX-M-14 on an IncK plasmid. Conclusions The prevalence of ESBL/AmpC-producing E. coli decreased over time. A clonal spread was observed on farm 2 and farm 3, illustrative of the complex dynamics probably associated with the use of antimicrobials.
- Subjects
BETA lactamases; ESCHERICHIA coli; CALVES; PLASMIDS; DRUG resistance
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2013, Vol 68, Issue 11, p2468
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkt219