We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Meffil: efficient normalization and analysis of very large DNA methylation datasets.
- Authors
Min, J L; Hemani, G; Smith, G Davey; Relton, C; Suderman, M
- Abstract
Motivation DNA methylation datasets are growing ever larger both in sample size and genome coverage. Novel computational solutions are required to efficiently handle these data. Results We have developed meffil, an R package designed for efficient quality control, normalization and epigenome-wide association studies of large samples of Illumina Methylation BeadChip microarrays. A complete re-implementation of functional normalization minimizes computational memory without increasing running time. Incorporating fixed and random effects within functional normalization, and automated estimation of functional normalization parameters reduces technical variation in DNA methylation levels, thus reducing false positive rates and improving power. Support for normalization of datasets distributed across physically different locations without needing to share biologically-based individual-level data means that meffil can be used to reduce heterogeneity in meta-analyses of epigenome-wide association studies. Availability and implementation https://github.com/perishky/meffil/ Supplementary information Supplementary data are available at Bioinformatics online.
- Subjects
DNA methylation; EPIGENOMICS; META-analysis; GENE expression; METHYLATION
- Publication
Bioinformatics, 2018, Vol 34, Issue 23, p3983
- ISSN
1367-4803
- Publication type
Article
- DOI
10.1093/bioinformatics/bty476