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- Title
Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier.
- Authors
Lukondeh, Tredwell; Ashbolt, Nicholas J.; Rogers, Peter L.
- Abstract
Mannoprotein with emulsification properties was extracted from the cell walls of Kluyveromyces marxianus grown on a lactose-based medium by autoclaving cells in a citrate buffer at pH 7.The purified product was evaluated for chemical and physical stability to establish its potential use as a natural emulsifier in processed foods. The yield of purified bioemulsifier from this strain of K. marxianus was 4–7% of the original dry cell weight. The purified product, at a concentration of 12 g 1-1, formed emulsions that were stable for 3 months when subjected to a range of pH (3–11) and NaCl concentrations (2–50 g 1-1). The composition of this mannoprotein was 90% carbohydrate (mannan) and 4–6% protein. These values are similar to mannoprotein extracted from cells of Saccharomyces cerevisiae, which is the traditional source. Consequently K. marxianus cultivated on a low-cost lactose-based medium such as whey, a lactose-rich clean waste of the dairy industry, could be developed as a source of bioemulsifier for use in the food industry.
- Subjects
KLUYVEROMYCES marxianus; CITRATES; PROCESSED foods; EMULSIONS; CARBOHYDRATES; SACCHAROMYCES cerevisiae; LACTOSE; DAIRY industry; FOOD industry
- Publication
Journal of Industrial Microbiology & Biotechnology, 2003, Vol 30, Issue 12, p715
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-003-0105-6