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- Title
Registration of Changes in the Level of Extracellular Dopamine in the Nucleus Accumbens by Fast-Scan Cyclic Voltammetry during Stimulation of the Zone of the Ventral Tegmentаl Area, Which Also Caused a Self-Stimulation.
- Authors
Pyurveev, S. S.; Sizov, V. V.; Lebedev, A. A.; Bychkov, E. R.; Mukhin, V. N.; Droblenkov, A. V.; Shabanov, P. D.
- Abstract
The aim of the work was to establish the possibility of using fast-scan cyclic voltammetry (FSCV) method of recording of dopamine release in the nucleus accumbens in response to electrical stimulation of that zone of ventral tegmental area (VTA) which used to study the possibility of forming a self-stimulation response in rats. Fast-scan cyclic voltammetry in vivo was used to record the release of dopamine in the nucleus accumbens in response to electrical stimulation of the VTA, and then the self-stimulation reaction of the same VTA zones was worked out in these animals. At the first stage, the anesthetized animals were stimulated (240 µA) with a burst of pulses with self-stimulation parameters (100 Hz), lasting 0.5 s every 3 min for 1 h. Then phenamine (1 mg/kg) was injected, and recording was continued for 1 hour. VTA electrical stimulation caused an increase in the content of extracellular dopamine for 1.45 ± 0.03 seconds with maximum amplitude of 4.6 ± 0.3 nA. The administration of phenamine during stimulation of the zone of positive reinforcement increased both the duration of the phasic dopamine reaction to 5.2 ± 0.1 seconds and the amplitude of this up to 20.8 ± 0.9 nA, which corresponds to a proportional increase in the amount of DA. This response was maximally expressed at the 45th minute after injection of phenamine. The investigated VTA zones were also tested for the suitability for a self-stimulation response formation in the "rise" test, when the instrumental reaction for intracerebral reinforcement consisted in raising the head by 38 degrees in a wireless telemetry unit. The self-stimulation response was tested before the administration of phenamine and after that for 1 hour. It was found that the number of rises to receive intracerebral reinforcement increased by 45 minutes after the administration of phenamine. Thus, the present study showed that stimulation of zones of positive reinforcement in the VTA in anesthetized animals causes persistent, long-lasting effects of phasic dopamine release. Activation of dopamine release after phenamine administration corresponds in time to changes in self-stimulation. In addition, the combination of the FSCV method and the self-stimulation makes it possible to localize the stimulating electrode in the VTA more clearly and reliably, under the control of dopamine release registration. This gives grounds for the application of this method, in the form of two experiments: (1) registration of dopamine release using fast-scan cyclic voltammetry in anesthetized animals, and (2) study of the self-stimulation response. This approach holds promise for standardizing protocols for evaluating the effects of sensory and pharmacological agents on dopamine-dependent behaviors.
- Subjects
DOPAMINE receptors; NUCLEUS accumbens; CYCLIC voltammetry; DOPAMINE; ELECTRIC stimulation; RECORDING &; registration
- Publication
Journal of Evolutionary Biochemistry & Physiology, 2022, Vol 58, Issue 5, p1613
- ISSN
0022-0930
- Publication type
Article
- DOI
10.1134/S0022093022050295