We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Misaponin B Induces G2/M Arrest, Cytokinesis Failure and Impairs Autophagy.
- Authors
Won, Gunho; Jung, Ji Hoon; Sohn, Eun Jung; Park, Ji Eon; Kim, Hyungjin; Lee, Hyo-Jung; Shim, Bum Sang; Kim, Sung-Hoon
- Abstract
Saponins are a group of naturally occurring plant glycosides with the features of their strong foam-forming properties and multibiological effects such as antitumor activity. Though Misaponin B, one of the triterpenoid saponins from Madhuca longifolia, is known to have spermicidal and antioxidant activity, the other biological activities have been never reported so far. Thus, in the present study, the antitumor mechanism of Misaponin B was investigated in A549 and AsPC-1 cancer cells. Misaponin B exerted significant cytotoxicity in A549, H460, SKOV3, and AsPC-1 cancer cells. Among them, A549 and AsPC-1 cells were more susceptible to Misaponin B. Misaponin B induced G2/M arrest and cytokinesis failure and increased the expression of LC3B and p62 with autophagic vacuoles and GFP-LC3 punctae in A549 and AsPC-1 cells. Furthermore, Misaponin B suppressed autophagy flux in A549 cells transfected by GFP-mRFP-LC3 constructs by showing merged yellow color by autophagy flux assay. Overall, our findings provide evidences that Misaponin B induces G2M arrest and impairs autophagy in A549 and AsPC-1 cells.
- Subjects
AUTOPHAGY; CELL cycle; CELL lines; CELL physiology; CYTOSKELETAL proteins; GENE expression; GLYCOSIDES; MOLECULAR structure; TUMORS
- Publication
BioMed Research International, 2020, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2020/5925094