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- Title
A crossreactivity at the immunoglobulin E level of the cell wall mannoproteins of Candida albicans with other pathogenic Candida and airborne yeast species.
- Authors
Kanbe, T.; Utsunomiya, K.; Ishiguro, A.
- Abstract
Background Candida albicans crossreacts with Sacchormyces cerevisiae or Pityrosporum male at the IgE level. However, the extent of crossreactivity of C. albicans with other yeast species is not known. Objective The crossreactivity at the immunoglobulin E (IgE) level of Candida albicans with other pathogenic Candida species and to the airborne yeast species Crptococcus and Rhodotorula was studied by immunoblot analysis. Methods Crude antigens. designated as heat extract, were prepared from 13 different yeast species and a dot blot test was performed to detect IgE antibodies against each of the heat extracts in 349 patients with allergies who were positive for IgE antibodies against C. albicans in a CAP system. Results In the dot blot test, most of the sera reacted with the heat extracts of not only C. albicans but also those prepared from the other yeast species. The sera of 41 of the 349 patients (11 .7%) reacted with the heat extracts of all 13 yeast species. The extent of the binding of IgE antibodies to multiple yeast species correlated with both the fluorescence intensities measured in the CAP system arid the intensities of dots generated by the heat extract of C. albicans in the clot blot test. In an inhibition dot blot test. In an inhibition dot blot test, mannoproteins, but not proteins, of C. albicans strongly inhibited the subsequent binding of IgE antibodies to all yeast species. Conclusion Our data suggest that the C. albicans mannoproteins are responsible for the crossreactivity among these yeast species at the IgE level.
- Subjects
IMMUNOGLOBULINS; CANDIDIASIS; EXTRACTS; CANDIDA albicans; ALLERGIES; BLOOD plasma
- Publication
Clinical & Experimental Allergy, 1997, Vol 27, Issue 12, p1449
- ISSN
0954-7894
- Publication type
Article
- DOI
10.1111/j.1365-2222.1997.tb02990.x