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- Title
The specific expression of Hypoxia Inducible Factor-1α in human gastric mucosa induced by nonsteroidal anti-inflammatory drugs.
- Authors
Ito, M.; Tanaka, S.; Kim, S.; Kuwai, T.; Matsutani, N.; Kamada, T.; Kitadai, Y.; Sumii, M.; Yoshihara, M.; Haruma, K.; Chayama, K.
- Abstract
Summary Background : Hypoxia is a cause of gastric mucosal damage induced by nonsteroidal anti-inflammatory drugs (NSAIDs). The expression of hypoxia inducible factor-1α (HIF-1α) reflects the status of tissue ischaemia. Aim : To investigate the effect of NSAID administration on the expression of HIF-1α in human gastric mucosa. Methods : We employed 71 patients including 14 with NSAID administration. The HIF-1α expression was estimated by immunohistochemistry using monoclonal antibody (H1α67) and raised antiserum (HI-3). Vascular endothelial growth factor expression was also examined by immunohistochemistry. HI-3 recognized hypoxia-induced protein in HeLa cells. Results : In human gastric mucosa, HIF-1α was mainly expressed in the nuclei of the surface epithelial cells and in the neck zone both by use of HI-3 and of H1α67. The expression of vascular endothelial growth factor correlated well with that of HIF-1α. The level of HIF-1α in the surface epithelium was significantly higher in patients with administration of NSAIDs than those without NSAID use (P < 0.001) both in the gastric corpus and antrum. Helicobacter pylori infection did not affected the levels of HIF-1α. Long-term administration of rebamipide reduced the level of HIF-1α. Conclusion : HIF-1α expression is a new biological marker of ischaemia especially in NSAID-related gastric lesions.
- Subjects
HYPOXEMIA; GASTRIC mucosa; NONSTEROIDAL anti-inflammatory agents; WOUNDS &; injuries
- Publication
Alimentary Pharmacology & Therapeutics, 2003, Vol 18, p90
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1046/j.1365-2036.18.s1.10.x