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- Title
Synthesis of Mono- and Dihydroxylated Amino Acids with New α-Ketoglutarate-Dependent Dioxygenases: Biocatalytic Oxidation of CH Bonds.
- Authors
Baud, Damien; Saaidi, Pierre‐Loïc; Monfleur, Adam; Harari, Marine; Cuccaro, Julien; Fossey, Aurélie; Besnard, Marielle; Debard, Adrien; Mariage, Aline; Pellouin, Virginie; Petit, Jean‐Louis; Salanoubat, Marcel; Weissenbach, Jean; de Berardinis, Véronique; Zaparucha, Anne
- Abstract
Iron(II)/α-ketoacid-dependent oxygenases (αKAOs) are enzymes that mainly catalyse hydroxylation reaction. By using a genomic approach combining sequence comparison and protein-domain sharing, a set of 131 αKAO enzymes was prepared. The screening of various substrates revealed five new αKAOs. Four αKAOs were found to be active towards L-lysine, L-ornithine and L-arginine with total regio- and stereoselectivities and yielding the corresponding 3- or 4-hydroxyamino acids. The enzymatic cascade reaction with two stereoselective regiodivergent αKAOs enabled the synthesis of 3,4-dihydroxy- L-lysine.
- Subjects
AMINO acids; BIOCATALYSIS; DIOXYGENASES; HYDROXYLATION; REGIOSELECTIVITY (Chemistry)
- Publication
ChemCatChem, 2014, Vol 6, Issue 10, p3012
- ISSN
1867-3880
- Publication type
Article
- DOI
10.1002/cctc.201402498