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- Title
Simplified methods to isolate, culture and purify olfactory ensheating cells.
- Authors
Zhengfeng Lu; Yixin Shen; Peng Zhang; Zhihai Fan; Qirong Dong; Min Wang
- Abstract
The article reports on simplified methods for isolating, culturating, purifying olfactory, ensheathing cells. The isolation of olfactory ensheathing cells was conducted using shearing, dispersion processes. A three-step purification procedure was then performed after the primary cultures attained confluence. Cells attached and developed quickly. The three-step purification process allows for the cells to be preserved for extended periods of time in culture.
- Subjects
CELLS; OLFACTORY nerve; DISPERSION (Chemistry); CELL culture
- Publication
Neural Regeneration Research, 2010, Vol 5, Issue 19, p2
- ISSN
1673-5374
- Publication type
Article