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- Title
Induction of Th2 cytokine expression for p27-specific IgA B cell responses after targeted lymph node immunization with simian immunodeficiency virus antigens in rhesus macaques.
- Authors
Kawabata, Shigetada; Miller, Christopher J.; Lehner, Thomas; Fujihashi, Kohtaro; Kubota, Mitsuru; McGhee, Jerry R.; Imaoka, Koichi; Hiroi, Takachika; Kiyono, Hiroshi; Kawabata, S; Miller, C J; Lehner, T; Fujihashi, K; Kubota, M; McGhee, J R; Imaoka, K; Hiroi, T; Kiyono, H
- Abstract
To determine if there is an association between the isotype of simian immunodeficiency virus (SIV)-specific B cell responses and the profile of Th1 and Th2 cytokine expression, rhesus macaques were immunized with SIV antigens via the iliac lymph nodes, using a targeted lymph node (TLN) immunization procedure. When CD4+ T cells purified from antigen-stimulated peripheral blood mononuclear cells were analyzed, the levels of Th2 cytokine production were gradually increased after the second and third immunizations. However, interferon-gamma production did not change. Analysis of SIV-specific B cell responses revealed that the main isotype was IgG after the second and third immunizations. In addition, a peak of SIV-specific IgA B cell responses was noted following the third immunization. These findings suggest that the induction of Th2 type responses in TLN-immunized rhesus macaques reflects the sequence of initial induction of SIV-specific IgG-producing cells followed by IgA-secreting cells.
- Publication
Journal of Infectious Diseases, 1998, Vol 177, Issue 1, p26
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1086/513811