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- Title
Detection of AmpC β-lactamase producing bacteria isolated in neonatal sepsis.
- Authors
Salamat, Sonia; Ejaz, Hasan; Zafar, Aizza; Javed, Humera
- Abstract
Objective: The objective of this study was to determine the occurrence and antimicrobial profile of AmpC β-lactamase producing bacteria. Methods: The study was conducted at The Children s Hospital and The Institute of Child Health Lahore, Pakistan, during September 2011 to June 2012. A total number of 1,914 blood samples of suspected neonatal septicemia were processed. Isolates were identified using Gram s staining, API 20E and API 20NE tests. Gram negative isolates were screened for AmpC β-lactamase production against ceftazidime, cefotaxime and cefoxitin resistance and confirmed by inhibitor based method. Results: Total number of 54 (8.49%) Gram positive and 582 (91.5%) Gram negative bacteria were identified. Among Gram negative isolates 141 (22%) were AmpC producers and found to be 100% resistant to co-amoxiclav, cefoxitin, ceftazidime, cefotaxime, cefuroxime, cefixime, ceftriaxone, cefpodoxime, gentamicin, amikacin and aztreonam. Less resistance was observed against cefepime (30.4%), sulbactamcefoperazone (24.8%), piperacillin-tazobactam (10.6%), ciprofloxacin (20.5%) and meropenem (2.1%). All the isolates were found sensitive to imipenem. The patients harbored AmpC β-lactamases were on various interventions in which intravenous line was noted among (51.1%), naso-gastric tube (37.6%), ambu bag (8.5%), endotracheal tube (3.5%), ventilator (2.1%) and surgery (0.7%). Conclusion: Extensive use of invasive procedures and third generation cephalosporins should be restricted to avoid the emergence of AmpC beta-lactamases in neonates.
- Subjects
DIAGNOSIS of neonatal diseases; NEONATAL sepsis; ANTI-infective agents; BETA lactamases; CEFOTAXIME; THERAPEUTICS
- Publication
Pakistan Journal of Medical Sciences, 2016, Vol 32, Issue 6, p1
- ISSN
1682-024X
- Publication type
Article
- DOI
10.12669/pjms.326.10861