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- Title
Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana.
- Authors
Guo, Pingan; Zheng, Yancheng; Chen, Jie; Wang, Bo; Liu, Lijun; Feng, Enying; Peng, Dingxiang
- Abstract
The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named BnGDSpro and BnSTMpro) from ramie and analyzed their functions in Arabidopsis. The results show that BnGDSpro and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while BnSTMpro and its 5′-truncated fragments have apical meristem specificity in Arabidopsis seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of BnSTMpro are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO4 treatment reveals that both BnGDSpro and BnSTMpro are copper-inducible promoters. Our findings suggest that BnGDSpro and BnSTMpro and their truncated fragments have potential applications in plant genetic transformation.
- Subjects
PROMOTERS (Genetics); RAMIE; ARABIDOPSIS thaliana; MERISTEMS; PLANT genetic engineering
- Publication
Plant Cell, Tissue & Organ Culture, 2019, Vol 136, Issue 3, p467
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-018-01528-9