We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Association of waxy gene single nucleotide polymorphisms with starch characteristics in rice (Oryza sativa L.).
- Authors
Larkin, Patrick D.; Park, William D.
- Abstract
The waxy gene, which encodes the granule bound starch synthase enzyme, is one of the key genes influencing starch synthesis in the rice endosperm. To investigate functional differences between GBSS alleles, we cloned and sequenced GBSS cDNA from a series of cultivars that differed substantially in apparent amylose content and starch viscosity characteristics. We found two single nucleotide polymorphisms in exons 6 and 10 that resulted in amino acid substitutions. These substitutions are associated with differences in apparent amylose content and viscosity characteristics. Subsequent sequencing of these regions from additional cultivars confirmed their association with particular rice quality characteristics. These point mutations could prove useful as molecular markers in the production of cultivars with superior eating, cooking and processing quality, and contribute to our understanding of the various structural and functional differences among granule bound starch synthase alleles.
- Subjects
GENES; STARCH synthesis; ENZYMES; DNA; AMINO acids; RICE
- Publication
Molecular Breeding, 2003, Vol 12, Issue 4, p335
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1023/B:MOLB.0000006797.51786.92