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- Title
Synergistic effects of TAL1 over-expression and PHO13 deletion on the weak acid inhibition of xylose fermentation by industrial Saccharomyces cerevisiae strain.
- Authors
Li, Yun-Cheng; Gou, Zi-Xi; Liu, Ze-Shen; Tang, Yue-Qin; Akamatsu, Takashi; Kida, Kenji
- Abstract
In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete the gene encoding p-nitrophenylphosphatase ( PHO13) and overexpress the gene encoding transaldolase ( TAL1) to evaluate the synergistic effects of these two genes on xylose fermentation in the presence of weak acid inhibitors, including formic, acetic, or levulinic acids. TAL1 over-expression or PHO13 deletion improved xylose fermentation as well as the tolerance of NAPX37 to all three weak acids. The simultaneous deletion of PHO13 and the over-expression of TAL1 had synergistic effects and improved ethanol production and reduction of xylitol accumulation in the absence and presence of weak acid inhibitors.
- Subjects
TRANSALDOLASE; GENETIC code; SACCHAROMYCES cerevisiae; GENE expression; PHOSPHATASES; XYLOSE; FERMENTATION; BIOMASS
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 10, p2011
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1581-7