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- Title
A simple structured model for recombinant IDShr protein production in Pichia pastoris.
- Authors
Mendoza Muñoz, Diego; Algecira Enciso, Néstor; Córdoba Ruiz, Henry; Barrera Avellaneda, Luis
- Abstract
A simple structured model is proposed for the methanol production phase of the iduronate 2-sulphate sulfatase recombinant enzyme (IDShr) in Pichia patoris Mut+. The model is mainly focused in oxidative stress phenomenon due to methanol consumption and based on extracellular experimental information and the basic knowledge of methanol metabolism in Pichia pastoris yeast ( P. pastoris). The model’s prediction shows a reasonable accuracy as compared with the experimental data. Likewise, it was proved that this model is able to simulate the production of other recombinant protein in P. pastoris.
- Subjects
PROTEINS; PICHIA pastoris; SULFATES; ENZYMES; METHANOL; YEAST
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 10, p1727
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9750-1