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- Title
Efficacy of In Vivo Electroporation-Mediated IL-10 Gene Delivery on Survival of Skin Flaps.
- Authors
Seyed Jafari, S. Morteza; Shafighi, Maziar; Beltraminelli, Helmut; Weber, Benedikt; Schmid, Ralph. A.; Geiser, Thomas; Gazdhar, Amiq; Hunger, Robert E.
- Abstract
Despite advances in understanding the underlying mechanisms of flap necrosis and improvement in surgical techniques, skin flap necrosis after reconstructive surgery remains a crucial issue. We investigated the efficacy of electroporation-mediated IL-10 gene transfer to random skin flap with an aim to accelerate wound healing and improve skin flap survival. Nine male Wistar rats (300-330 g) were divided in two groups (a) control group (n = 5), only surgery no gene transfer, and (b) experimental group, received electroporation-mediated IL-10 gene transfer 24 h before the surgery as prophylaxis (n = 4). Random skin flap (McFarlane) was performed in both groups. Planimetry, Laser Doppler imaging, and immunohistochemistry were used to evaluate the effect of IL-10 gene transfer between study groups at day 7. Electroporation-mediated IL-10 gene transfer decreased percentage of flap necrosis (p value = 0.0159) and increased cutaneous perfusion compared to the control group (p value = 0.0159). In addition, Spearman's rank correlation showed a significant negative correlation between percentage of flap necrosis and Laser Index (p value = 0.0083, r -0.83, respectively). Furthermore, significantly higher mean CD31+ vessel density was detected in the experimental group compared to the control group (p value = 0.0159). Additionally, semi-quantitative image analysis showed lower inflammatory cell count in experimental group compared to control group (p value = 0.0317). In vivo electroporation-mediated IL-10 gene transfer reduced necrosis, enhanced survival and vascularity in the ischemic skin flap.
- Subjects
INTERLEUKIN-10; ELECTROPORATION; NECROSIS; GENE therapy; INFLAMMATION; GENETIC transformation; ANIMAL experimentation; COMPARATIVE studies; CYTOLOGY; SURGICAL flaps; INTERLEUKINS; RESEARCH methodology; MEDICAL cooperation; RATS; RESEARCH; SKIN grafting; WOUND healing; EVALUATION research
- Publication
Journal of Membrane Biology, 2018, Vol 251, Issue 2, p211
- ISSN
0022-2631
- Publication type
journal article
- DOI
10.1007/s00232-017-9974-x