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- Title
Salinity-Induced Noise in Membrane Potential of Characeae Chara australis: Effect of Exogenous Melatonin.
- Authors
Beilby, Mary; Al Khazaaly, Sabah; Bisson, Mary
- Abstract
Salt sensitive Characeae Chara australis responds to 50 mM NaCl by a prompt appearance of noise in the trans-membrane potential difference (PD). The noise diminishes with time in saline and PD depolarization, leading to altered current-voltage characteristics that could be modeled with H/OH channels. Beilby and Al Khazaaly (JMB 230:21-34, ) suggested that the noise might arise from cooperative transient opening of H/OH channels. Presoaking cells in 10 μM melatonin over 24 h abolished the noise in some cells, postponed its appearance in others or changed its characteristics. As melatonin is a very effective antioxidant, we postulated opening of H/OH channels by reactive oxygen species (ROS). Measurement of ROS using dihydrodichlorofluorescein diacetate confirmed substantial reduction in ROS production in melatonin-treated cells in saline and sorbitol media. However, ROS concentration decreased as a function of time in saline medium. Possible schemes for activation of H/OH channels under salinity stress are considered.
- Subjects
SALINITY; MEMBRANE potential; MELATONIN; NOISE; SORBITOL
- Publication
Journal of Membrane Biology, 2015, Vol 248, Issue 1, p93
- ISSN
0022-2631
- Publication type
Article
- DOI
10.1007/s00232-014-9746-9