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- Title
Expression of inhibin α, glial cell line-derived neurotrophic factor and stem cell factor in Sertoli cell-only syndrome: relation to successful sperm retrieval by microdissection testicular sperm extraction.
- Authors
K. Fujita; A. Tsujimura; T. Takao; Y. Miyagawa; K. Matsumiya; M. Koga; M. Takeyama; H. Fujioka; K. Aozasa; A. Okuyama
- Abstract
BACKGROUND: Microdissection testicular sperm extraction (TESE) has provided new hope for successful sperm retrieval to patients with Sertoli cell-only syndrome (SCO). We determined expression of the inhibin a subunit, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in Sertoli cells obtained from patients with SCO immunohistochemically and compared expression rates with rates of microdissection TESE sperm retrieval. METHODS: Testicular biopsy specimens were obtained from 52 men with non-obstructive azoospermia who underwent microdissection TESE and were diagnosed with SCO by histological analysis. RESULTS: All specimens showed intense staining for the inhibin a subunit. Moderate or intense staining for GDNF was observed in 65.8% of specimens. All but one showed moderate or intense staining for SCF. Among specimens negative for GDNF, the sperm retrieval rate was significantly higher (100%) for specimens with intense staining for SCF than for specimens with no or moderate staining (30.7%) (P<0.05) for SCF. CONCLUSION: GDNF expression differs among patients with SCO. The sperm retrieval rate was high in cases of no staining for GDNF and intense staining for SCF.
- Subjects
MICRODISSECTION; CELLS; INHIBIN; SERTOLI cells
- Publication
Human Reproduction, 2005, Vol 20, Issue 8, p2289
- ISSN
0268-1161
- Publication type
Article
- DOI
10.1093/humrep/dei032